American Society of Hematology

Fig. 2.

$Fig. 2. Expression of p21WAF1 mRNA in γ-irradiated OCI/AML-5 cells. Samples of total RNA (20 μg) prepared from cells at different times after exposure to 6 Gy of γ-irradiation were fractioned on an agarose-formaldehyde gel, transferred to a nylon membrane, and hybridized sequentially with 32P-labeled probes for human p21WAF1 cDNA (A) and 18S ribosomal RNA (B). OCIM2 cells, which express mutant p53 protein, were used as a control. Signal intensities were quantitated on a phosphorimager. The ratio of the p21WAF1 RNA signal to the 18S ribosomal RNA signal in the OCIM2 sample (0 hours) was arbitrarily set to 1.0 and the normalized values of p21WAF1 mRNA are shown at the bottom of (A).$

Expression of p21^WAF1 mRNA in γ-irradiated OCI/AML-5 cells. Samples of total RNA (20 μg) prepared from cells at different times after exposure to 6 Gy of γ-irradiation were fractioned on an agarose-formaldehyde gel, transferred to a nylon membrane, and hybridized sequentially with ³²P-labeled probes for human p21^WAF1 cDNA (A) and 18S ribosomal RNA (B). OCIM2 cells, which express mutant p53 protein, were used as a control. Signal intensities were quantitated on a phosphorimager. The ratio of the p21^WAF1 RNA signal to the 18S ribosomal RNA signal in the OCIM2 sample (0 hours) was arbitrarily set to 1.0 and the normalized values of p21^WAF1 mRNA are shown at the bottom of (A).

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