Flow cytometric comparison of c7E3 and fibrinogen binding as well as P-selectin expression on resting and activated platelets and evaluation of the expression level and c7E3 binding of CHO cells transfected with recombinant GP IIb/IIIa, either in the activated or nonactivated state. (A) Binding of various concentrations of biotinylated c7E3 to ADP-stimulated and resting platelets are expressed as the mean ± standard deviation of three determinations. (B) Histogram of FITC-conjugated anti–P-selectin MoAb binding to ADP-stimulated and resting platelets. (C) Histogram of FITC-conjugated polyclonal antifibrinogen chicken Ab binding to ADP-stimulated and resting platelets. (D) Binding of biotinylated c7E3 to CHO cells expressing no, activated, or nonactivated GP IIb/IIIa. Results are expressed as the mean ± standard deviation of three determinations. (E) Histograms of FITC-conjugated MoAb P2 (anticomplex GP IIb/IIIa) with GP IIb/IIIa (in the activated and nonactivated state) expressing CHO cell lines, together with CHO cells as a negative control. Typical histograms of several determinations are depicted.