Inhibition of NF-κB activation blocks LPS-mediated A1 and A20 induction. (A) HMEC-1 cells transduced with a FLAG-IκBmt construct (HMEC-IκBmt) or empty vector (HMEC-Neo) were treated with LPS or TNF for 3 hours and subjected to Northern analysis. (B) Transduced cell lines were immunoblotted with the FLAG (M2) antibody to confirm expression of the FLAG-IκBmt protein.