LPS-initiated accumulation of A1 and A20 mRNA is an early gene response dependent on CD14. (A) Northern analysis of HMEC-1 cells treated with neutralizing antibodies to CD14 and TNF, an IL-1 antagonist (IL-1RA), or an anti-CD14 isotype control (IgG2a) for 1 hour before stimulation with LPS (100 ng/mL) for 3 hours. (B) Northern analysis of HMEC-1 cells after 3 hours of treatment with LPS (100 ng/mL) only, CHX (50 μg/mL) only, or LPS and CHX.