Fig. 1.
Fig. 1. RT-PCR amplification of IgH VDJ using consensus primers from single BM plasma cells of a myeloma patient. (A) Individual BM plasma cells from patient 2 were purified, stained, and sorted into PCR tubes containing lysis buffer, followed by reverse transcription and seminested amplification of IgH VDJ mRNA using consensus primers to FR2 and Jh as indicated in methods. Product was amplified from 10 of 14 plasma cells (lines 1-14) (M: molecular weight marker − 100 bp ladder). (B) Individual BM plasma cells from patient 2 were purified, stained, and sorted into PCR tubes containing lysis buffer, followed by reverse transcription and nested amplification of IgH VDJ mRNA first using consensus primers to FR2 and Jh and next using specific primers to CDR2 and CDR3 regions of clonotypic IgH for patient 2. Product was amplified from 12 of 14 plasma cells (lines 1-14) (M: molecular weight marker − 100 bp ladder).

RT-PCR amplification of IgH VDJ using consensus primers from single BM plasma cells of a myeloma patient. (A) Individual BM plasma cells from patient 2 were purified, stained, and sorted into PCR tubes containing lysis buffer, followed by reverse transcription and seminested amplification of IgH VDJ mRNA using consensus primers to FR2 and Jh as indicated in methods. Product was amplified from 10 of 14 plasma cells (lines 1-14) (M: molecular weight marker − 100 bp ladder). (B) Individual BM plasma cells from patient 2 were purified, stained, and sorted into PCR tubes containing lysis buffer, followed by reverse transcription and nested amplification of IgH VDJ mRNA first using consensus primers to FR2 and Jh and next using specific primers to CDR2 and CDR3 regions of clonotypic IgH for patient 2. Product was amplified from 12 of 14 plasma cells (lines 1-14) (M: molecular weight marker − 100 bp ladder).

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