Fig. 2.
Fig. 2. Inhibition of autologous hematopoietic colony growth by CML CIK cells. 1 × 105 myeloid progenitor cells were incubated for 4 hours with autologous CIK cells at ratios of 1:1, 2:1, and 5:1; washed once; and then replated in 1mL of methyl cellulose. Each incubation was performed in triplicate, and colonies were scored after 14 days’ incubation in 5% CO2. Results are expressed as the percentage growth compared with control target cells incubated in medium alone. The mean number of colonies for 1 × 105 targets alone were MR-325, MBA-21, RG-35, and AZ-73. Patient M.R. grew 17 colonies/105 CIK cells, but this was not adjusted for in calculating the percentage growth compared to targets alone.

Inhibition of autologous hematopoietic colony growth by CML CIK cells. 1 × 105 myeloid progenitor cells were incubated for 4 hours with autologous CIK cells at ratios of 1:1, 2:1, and 5:1; washed once; and then replated in 1mL of methyl cellulose. Each incubation was performed in triplicate, and colonies were scored after 14 days’ incubation in 5% CO2. Results are expressed as the percentage growth compared with control target cells incubated in medium alone. The mean number of colonies for 1 × 105 targets alone were MR-325, MBA-21, RG-35, and AZ-73. Patient M.R. grew 17 colonies/105 CIK cells, but this was not adjusted for in calculating the percentage growth compared to targets alone.

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