The demonstration of the inhibition of the binding of soluble human FN to MK by Western blotting: Guinea pig MK were incubated with human plasma FN in solution at 37°C for 2 hours. FN bound to the MK surface was extracted, separated by SDS-PAGE, and visualized by ECL on Western blots with an antibody specific to human FN, IST-4. The figure shows a representative of four experiments in which MK were incubated with MK0852 (10 μmol/L; lane 1), bitistatin (2 μmol/L; lane 2), EMF10 (50 nmol/L; lane 3), control without integrin blockers (lane 4), HP2/1 (10μg/mL; lane 5), and SAM-1 (10 μg/mL; lane 6). The densitometric analysis of the three experiments is given in the Results.