Fig. 3.
Fig. 3. The effect of 2.4G2 on differentiation of B-lineage precursor cells (B220+, IgM−, HSAhi). Fresh FACS-sorted B220+, IgM− bone marrow cells (left panel) were cocultured with bone marrow stromal cell line BMS2. IgM and HSA expression on B220+ cells after 72 hours of culture without (middle panel) or with 2.4G2 (25 μg/mL; right panel). Cultures with 2.4G2 showed an enhanced percentage of cells with IgM expression and increased amounts of IgM per cell.

The effect of 2.4G2 on differentiation of B-lineage precursor cells (B220+, IgM−, HSAhi). Fresh FACS-sorted B220+, IgM− bone marrow cells (left panel) were cocultured with bone marrow stromal cell line BMS2. IgM and HSA expression on B220+ cells after 72 hours of culture without (middle panel) or with 2.4G2 (25 μg/mL; right panel). Cultures with 2.4G2 showed an enhanced percentage of cells with IgM expression and increased amounts of IgM per cell.

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