Recombinant LRP fragments from region II. (A) Electrophoresis of fragments on a 13.5% reducing SDS polyacrylamide gel. Lane 1 contains prestained low molecular weight markers (Life Technologies). The remaining lanes (2 through 6) contain the LRP fragments in the following order: E4 (8.2 kD), E4-C3 (13.2 kD), C5-C7 (16.8 kD), E5-AA1399 (29.5 kD), and E4-C10 (44.8 kD). The smaller proteins migrate abnormally slowly and appear to be larger than the actual molecular weights shown in parentheses due to the presence of the 6-His tag. (B) RAP affinity-purified fragments on a nonreducing 13.5% SDS polyacrylamide gel. Lane 1 contains low molecular weight markers, lane 2 contains C5-C7, and lane 3 contains E4-C10. Both gels were stained with Coomassie Brilliant blue.