Clonal analysis for the populations of PEC-derived granulocytes and macrophages. The PEC isolated from the same mice described in Fig 2A were used for clonal analysis. Genomic DNA was prepared from PEC 24 and 96 hours after injection of thioglycollate and digested with the restriction enzyme BamHI or HindIII and probed using a neo-specific probe. Lanes 1, 3, 7, and 9 represent DNA from granulocytes (24 hours) and lanes 2, 4, 8, and 10 represent DNA from macrophages (96 hours) as indicated by the lettering below the lanes. Genomic DNA from the N2 producer cell (lanes 5 and 11) and 1 copy of plasmid DNA, pN2 (lanes 6 and 12), were used for oligoclonal and monoclonal controls, respectively. The last four lanes represent SacI-digested pN2 plasmid DNA equivalent to 0.125, 0.25, 0.5, and 1.0 copies/cell, respectively.
