LNGFR expression on human CD45⁺ cells obtained from the BM of NOD/SCID mice. NOD/SCID were sublethally irradiated and injected with LNSN-transduced CB-derived CD34⁺ cells (panels C through J) or mock-transduced cells (panels A and B). Six weeks later, BM cells were obtained from the femur of these animals and analyzed for the presence of human cells (CD45⁺) and LNGFR expression by FACS. FACS data from mice engrafted with cells derived from CB#1 (panels C through H) or CB#5 (panels I and J) is shown. The left panels show the CD45⁺expression versus the F(ab)-FITC isotype control. The right panels show the CD45 and LNGFR expression on LNSN-transduced (D through J) or mock-transduced (B) cells. Fluorescence intensities are displayed in logarithmic scale.