Multilineage engraftment of human hematopoietic cells into NOD/SCID mice. Sublethally irradiated NOD/SCID mice were injected with CB-derived CD34⁺ cells transduced for 3 days under serum-free conditions (CB#1 in Fig 2). (A) FACS analysis of hematopoietic cells obtained from the femur of animals injected with human CB-derived CD34⁺ cells (solid line) or control animals (dotted line). The percentage of human cells was calculated from the number of CD45⁺ cells found in the femur of the animals after 6 weeks. (B) Multilineage engraftment of human cells. The CD45⁺ cells shown in (A) were further analyzed for the presence of myeloid cells (CD13), lymphoid cells (CD19, CD2), and immature progenitor cells (CD34, CD38). The percentage of each cell population is shown in the upper right corner of each quadrant.