Comparative sequence analysis of rearranged V_H gene sequences from H-RS cells. Dashes indicate sequence identity. CDRs I-III are marked. Corresponding amino acids are shown above each codon. (A) PCR products of the V_H1 rearrangement amplified from 20 H-RS cells of a patient with NS-HD are compared with the germline V_H1 gene DP1432 and the J_H4 gene.31 The upper sequences were obtained from single H-RS cells (NSVH1), the lower sequences were obtained from lymph node tissues (LN 3/95 and LN 2/96). (B) V_H1 rearrangement amplified from eight H-RS cells from a patient with MC-HD, compared with the germline V_H1 gene DP1031 and the J_H3 gene.30 The upper sequences were obtained from single H-RS cells (MCVH1); the lower sequences were obtained from spleen (SP 7/95) and lymph node tissue (LN 7/96).