Effects of anti–IL-4R neutralizing Ab added at priming on the cytokine profile at restimulation. Cells were primed with anti-CD3, anti-OX40 (clone 315) or control mouse IgG2a, and CD32/B7.1 L cells in the presence of anti–IL-4R (5 μg/mL; ▧) or control mouse IgG2a (▧). Primed cells were restimulated for cytokine production as in the legend to Fig 2. Cells primed with anti-CD3 and CD32/B7.1 alone (□). Shown are the mean ± SEM of five experiments. *P < .05; **P < .01.