OX40 costimulation at priming deviates the cytokine production profile of primed T cells. T cells were primed either with anti-CD3 and anti-OX40 (clone 315) or control mouse IgG2a together with CD32/B7.1 L cells (A) or with anti-CD3 and OX40L L cells (B). In (C), T cells were cocultured with allogenic DC in the presence of anti-OX40L or control mouse IgG1. In each case, cells were restimulated for cytokine production after IL-2 expansion. IL-4, IL-5, and IFN-γ were measured after 24 hours and IL-13 after 48 hours of stimulation. Shown are the mean ± SEM of five experiments. *P < .05; **P < .01.