Northern blot analysis to examine CD164 expression in both hematopoietic progenitor and BM stromal cells. Total RNA derived from HBMSCs (lane 1) and the primitive myeloid cell line, KG1a (lane 2), were subjected to electrophoresis on a 1.0% formaldehyde-agarose gel and transferred to a nylon membrane by capillary action. CD164 mRNA expression was examined by overnight hybridization at 42°C with a³²P-radiolabeled full-length CD164 probe. The membrane was washed as described in Materials and Methods. Membranes were exposed to X-Omat AR film for 48 hours with intensifying screens. Hybridization to the 3.0-kb CD164 transcript is observed in both cell lines (indicated by arrow), whereas possible cross-hybridization with the 18s and 28s ribosomal RNA is indicated by open arrows.