Localization and expression of the siglec-5 gene. (A) chromosomal mapping by FISH: Human male metaphase chromosome spreads were hybridized with a 2-kb digoxygenin 11-dUTP-labeled HMQCD14 cDNA insert and the individual chromosomes were counterstained with DAPI. Digital images containing both DAPI bands and gene signal detected with anti-digoxygenin-tagged rhodamine are shown. The position of the siglec-5 gene on chromosome 19 is also shown schematically. (B) Northern blot analysis of siglec-5 mRNA in human tissues. Each lane of the Multiple Tissue Northern (MTN) Blot (Clontech) contains approximately 2 μg of poly A plus RNA from the tissues indicated and is normalized for levels of β-actin mRNA. Two major forms of siglec-5 mRNA are seen at approximately 2.4 and 3.4 kb. The hybridization pattern among the different tissue samples is consistent with expression of the siglec-5 gene being restricted to myelomonocytic cells.