Fig. 7.
Effect of suPAR-plasma suspension on the lysis of fibrin clots by scuPA/suPAR. An aliquot (10 μL) of the washed plasma/suPAR-Sepharose suspension (analyzed by SDS-PAGE in Fig 6) was added to 125I-labeled fibrin clots for 2 hours at 37°C and the release of radioactivity into the supernatant was measured. The first lane (solid bar) shows the activity of suPAR/scuPA on the fibrin clot. The second, middle lane, (open bar) shows the activity of scuPA/suPAR in the presence of the plasma/suPAR-Sepharose suspension. The third lane (hatched bar) shows the fibrinolytic activity of the plasma/suPAR-Sepharose suspension alone. The mean ± SEM of three experiments is shown.
