Fig. 4.
![Fig. 4. Ca2+ dependency of Rap1 activation. (A) Neutrophils were incubated with ionomycin (100 nmol/L) or thapsigargin (100 nmol/L) for the indicated time points. Rap1 GTP was detected as in the legend to Fig 1. (B) Ca2+-depleted neutrophils were stimulated with 1 μmol/L fMLP. As a control, non–Ca2+-depleted cells were stimulated with 1 μmol/L fMLP. Ca2+-depleted and untreated neutrophils of the same donor were taken to measure [Ca2+]i after 1 μmol/L fMLP stimulation. Representative examples of at least three independent experiments are shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/6/10.1182_blood.v92.6.2133/4/blod41819004w.jpeg?Expires=1724246927&Signature=XRDJa4e9UL4Jp5t5Ot~ejt5J03S1HRPZ-ukZZg16JxVJQjBYqA556r-0wkED-3bgybfOZH9a5eBIapue3SlZnGF32B3zthxr3RZybn8szyKfiO4ftAkO9TTjqJ6b5uLuCRrwoo4nxP36tGds0NeN61ueZyx54CXaS0LiWvTRTA3zXEMe0PbrHuLkXHmwqgCX-Qu3Ixe03Uomp4lxjOJr8Z1hpIX~lPy5~Fttb10cKA2S4uiHkPet87Ea8iLqVNgMp7XT4p-nneKxzYUS7TkVroIxrdJAu~Z12FLOCc9G-3K1idsR~XdBXdkT~yBdvnF55qIA-xhwu6TuI8UaZzF7ug__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Ca2+ dependency of Rap1 activation. (A) Neutrophils were incubated with ionomycin (100 nmol/L) or thapsigargin (100 nmol/L) for the indicated time points. Rap1 GTP was detected as in the legend to Fig 1. (B) Ca2+-depleted neutrophils were stimulated with 1 μmol/L fMLP. As a control, non–Ca2+-depleted cells were stimulated with 1 μmol/L fMLP. Ca2+-depleted and untreated neutrophils of the same donor were taken to measure [Ca2+]i after 1 μmol/L fMLP stimulation. Representative examples of at least three independent experiments are shown.
