Detection of vector sequences in PB from baboon T94433 transplanted with CD34-enriched marrow cells transduced by 72 hours of cocultivation (LNX) or over CH-296 (LN) both in the presence of IL-6, SCF, FLT3L, MGDF, and protamine sulfate. (A) PCR analysis of amplified vector sequences, LNX versus LN. (B) Percentage of vector-positive DNA measured by phosphorimage analysis of signal intensities for LN and LNX corrected for the amount of DNA as determined by actin PCR. (C) PCR analysis of T and B cells 12 and 16 weeks after transplantation. (D) Southern blot analysis of DNA from peripheral blood restricted withXba I, which cuts the plasmid forms of the vectors once in the 3’ LTR and cuts the integrated forms of the vectors once in each LTR, thus resulting in production of full-length vector fragment only from integrated vector. The respective fragment sizes are 2,369 bp (LN) and 2,394 bp (LNX).