Detection of vector sequences in peripheral blood from baboon J95009 transplanted with CD34-enriched marrow cells transduced by 72 hours of cocultivation (LNX) in the presence of IL-3, IL-6, and SCF or over CH-296 (LN) in the presence of IL-6, SCF, FLT3L, MGDF, and protamine sulfate. (A) PCR analysis of amplified vector sequences, LNX versus LN. Standards consist of single-vector copy HT1080/LN and HT1080/LNX DNA mixed at a 1:1 ratio and then mixed with normal baboon DNA in a log dilution series. PB, peripheral blood; BM, bone marrow. (B) Percentage of vector-positive DNA measured by phosphorimage analysis of signal intensities for LN (301 bp) and LNX (326 bp) corrected for the amount of DNA as determined by actin PCR.