EGMSA using GST-+-MITF fusion protein. The labeled 5’-GGAGGCCAGTCACTTGGTGAAGTC (oligo 1) was used as a probe (hexameric motif is shown by underlined and boxed by a solid line in the figure). The sequence of the oligonucleotide mutated in the CACTTG motif (to CTCTAG) is also shown as oligo 2. The mutated nucleotides are underlined. The excess amount of nonlabeled oligo 1 or oligo 2 was added as a competitor.