Adhesion of CMCs to VCAM-1. CMCs were used 4 weeks after the initiation of culture, because the expression of 4 subunit reached the maximum level at that time. The adhesion of CMCs to CHO cells transfected with either sense (CHO-VCAM-1) or antisense VCAM-1 cDNA (CHO-control) was determined. The adhesion of CMCs pretreated with anti-4 MoAb was also determined. Bars indicate the standard error of three assays.