Semiquantitative RT-PCR for the expression of various integrins in CMCs 4 weeks after the initiation of culture. RNAs obtained from +/+ CMCs (lanes 1 through 3) or from mi/miCMCs (lanes 4 through 6) were reverse-transcribed and PCR-amplified with 3, 4, 5, v, β1, β7, or β-actin primer. PCR products were electrophoresed in 2.0% agarose gel containing ethidium bromide. Amounts of RNA used for the reverse transcription were 5.0 μg (lanes 1 and 4), 0.5 μg (lanes 2 and 5), and 0.05 μg (lanes 3 and 6), respectively.