Effect of priming on cytogenetics in BM and PB. Marrow was obtained before priming and PB and BM were obtained after priming on protocols CY/GM/BM, MAC/G/PB, and CY/G/PB from all ECP and AP CML patients. Marrow and PB cells were evaluated by metaphase analysis to detect the number of Ph-positive metaphases. On average, 20 metaphases from steady-state marrow, mobilized marrow, or PB collections were analyzed. For PB collections, the average percent of Ph-positive metaphases in all collections was entered in the figure. Results are depicted for all samples that were analyzed and divided as follows: (□), major cytogenetic response, 0% to 30% of metaphases or cells Ph positive; (▧), minor cytogenetic response, 30% to 90% of metaphases or cells Ph positive; (▪), no response, 90% to 100% of metaphases or cells Ph positive.