Schematic diagram of the mutated FVII gene region and of the vectors used for the expression of mutant mRNA (1) and protein (2). The sequence of the Rsa I site is reported in bold; 2 and 6, primers. The open and closed triangles indicate the position of the IVS7 mutations and of the additional residues inserted in the FVII cDNA, respectively. P, Gla, EGF1, EGF2 and A, FVII domains.