Effect of AMG on granzyme B protein in NK cells. NK cells were cultured in RPMI complete medium during 12 hours in the absence (lane 1) or presence of AMG (lane 2) or with IL-12 + TNF (lane 3) and IL-12 + TNF + AMG (lane 4); the cells were then lysed. The cell lysate was analyzed by Western blot with an anti-granzyme B antibody, as described in Materials and Methods. Blot was quantitated by video densitometry and fold induction over the basal activity was indicated. Similar results were obtained in two other experiments.