RT-PCR analysis of PL scramblase in Scott and normal B lymphoblasts. RNA was extracted from three Scott (S0, S1, S2) and three normal control (C1, C2, W9) B-lymphoblast cell lines and RT-PCR performed with PL scramblase-specific primers. PCR products were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. See Materials and Methods for details. RT-PCR of β₂-microglobulin as control gene is also shown.