Northern blot analysis of PL scramblase in Scott and normal B lymphoblasts. RNA was extracted from Scott and normal control B lymphoblasts, and Northern blot performed with³²P-labeled PL scramblase cDNA probe as described in Materials and Methods. Results are shown for Scott S2 and normal control W9 (upper panel). Comparable amounts of transcripts were observed for all cell lines tested. Lower panel shows Northern blot of β-actin.