Schematic illustration of the localization of the primers for the nested SSP-PCR and representative results of FcγRIIa genotype of three individuals: homozygous FcγRIIa-R/R131 (R/R), heterozygous FcγRIIa-R/H131 (R/H), and homozygous FcγRIIa-H/H131 (H/H). For the FcγRIIa-specific amplification, primers P63 and P52 were used.27 For the allele-specific amplification, primers P5G (for the FcγRIIa-R131 allele) and P4A (for the FcγRIIa-H131 allele) were combined with a common antisense primer, P13. The 440-bp amplification product of CRP was used as an internal control. The 278-bp fragment represents the allele-specific amplification product from the SSP-PCR of FcγRIIa. Amplification with P13 and P5G (lane 1) and with P13 and P4A (lane 2) shows a homozygous FcγRIIa-R/R131 individual. Amplification with P13 and P5G (lane 3) and with P13 and P4A (lane 4) shows a heterozygous FcγRIIa-R/H131 individual. Amplification with P13 and P5G (lane 5) and with P13 and P4A (lane 6) shows a homozygous FcγRIIa-H/H131 individual. Lane 7 contains a molecular weight standard (100 bp).