Digestion of double-mutant cathepsin G with N-glycosidase F. RBL/CatG/Gly₂₀₁/▵Gly₁₉Glu₂₀cells were pulse-labeled with³⁵S-methionine/³⁵S-cysteine for 30 minutes, whereupon the label was chased for 3 hours. At each time point, 50 × 10⁶ cells were lysed and cathepsin G was immunoprecipitated. Half of the material was subjected to digestion with N-glycosidase F (indicated with “+”). Material without added N-glycosidase F, but otherwise treated identically, is shown as controls. The fluorogram was exposed for 5 days.