Fig. 1.
Monocyte-derived DC have potent APC function. DC prepared from adherent PBMC, myeloid-enriched non-T cells, or sorted monocytes (A), or from various sorted PBMC subpopulations (B), were cultured with 800 U/mL GM-CSF and 400 U/mL IL-4 for 4 days. Graded numbers (A) or 104 (B) mitomycin-treated APC were incubated with 105 allogeneic T cells for 5 days. Results are expressed as mean cpm of triplicate samples.

Monocyte-derived DC have potent APC function. DC prepared from adherent PBMC, myeloid-enriched non-T cells, or sorted monocytes (A), or from various sorted PBMC subpopulations (B), were cultured with 800 U/mL GM-CSF and 400 U/mL IL-4 for 4 days. Graded numbers (A) or 104 (B) mitomycin-treated APC were incubated with 105 allogeneic T cells for 5 days. Results are expressed as mean cpm of triplicate samples.

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