Effect of cycloheximide on the increase in Fas expression induced by anti-CD3 antibody or IFN-γ + IL-7. Fas expression was analyzed in human thymocytes after 24 hours of culture in control conditions or with anti-CD3 antibody or IFN-γ + IL-7 in the presence or absence of 10 μg/mL cycloheximide. (A) A representative analysis is presented. Thymocytes were first labeled with anti-Fas antibody, then with biotin-coupled antimouse antibody, and lastly with Quantum Red-conjugated streptavidine; only the last two steps were used for staining controls. Fas staining is shown as solid profiles and staining controls as open profiles. (B) The anti-CD3–induced increase in the Fashi cell proportion was not significantly modified by cycloheximide. (C) The cytokine-induced increase was fully inhibited by cycloheximide. Data are the means ± SEM of 5 experiments. (□) Medium; (⊟) IL-7 + IFN-γ.
