Western blot analysis for BSAP in hematolymphoid tumor cell lines. (A) YT, (B) Jurkat, (C) K562, (D) PB-697, (E) Nalm-6, (F) Ramos, (G) Granta 519, (H) SU-DHL-5, (I) SU-DHL-6, and (J) NCEB 1806. A 52-kD band of expected size is demonstrated only in the B-cell lines (D through J). PB-697 and Nalm-6 also display a second band of 46 kD, which has a much lower intensity in Nalm-6. This band originates from a cytoplasmic cross-reactive protein that does not appear to be related to BSAP (see text).