Functional analysis of mutations in the AT-rich sequence. HUVECs and HeLa cells were transfected as described in the legend to Fig 1. (A) The vectors pGvW(−89/+19), pGvW(−142/+19), and pGvW(−142m/+19) are depicted on the left side of the figures. The construct pGvW(−142m/+19) is mutated at nt −130 and −129 inside the AT-rich sequence. The CAT activities associated with these vectors are expressed relative to the activity of pGvW(−89/+19), which is set at 100%, and are given on the right. (▪) HUVECs; () HeLa cells. (B) The vectors pGvW(−89/+244), pGvW(−142/+244), and pGvW(−142m/+244) are shown on the left side of the panel. The CAT activities in HUVECs are expressed relative to the activity of the plasmid pGvW(−89/+19). The results are the mean ± SD of 4 to 6 experiments.