American Society of Hematology

Fig. 1.

Sensitivity of standard and nested RT-PCR assays for detection of MLL-AF4 fusion transcript-positive cells. Varying numbers (1 to 107) of MLL-AF4+ RS4;11 cells were mixed with cells from an EBV-transformed lymphoblastoid B-cell line to yield 0.00001% to 100%MLL-AF4+ cells in a test cell population containing a total of 107 cells. RNA was subtracted from these 107 cell samples and analyzed by standard RT-PCR as well as nested RT-PCR for MLL-AF4positivity, as described in Materials and Methods. CON, control.

Sensitivity of standard and nested RT-PCR assays for detection of MLL-AF4 fusion transcript-positive cells. Varying numbers (1 to 10⁷) of MLL-AF4⁺ RS4;11 cells were mixed with cells from an EBV-transformed lymphoblastoid B-cell line to yield 0.00001% to 100%MLL-AF4⁺ cells in a test cell population containing a total of 10⁷ cells. RNA was subtracted from these 10⁷ cell samples and analyzed by standard RT-PCR as well as nested RT-PCR for MLL-AF4positivity, as described in Materials and Methods. CON, control.

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