Fig. 6.
Analysis of fluorescently labeled stem cells in vitro. lin−CD34+Sca-1+c-Kit+fetal liver stem cells were stained with the fluorescent dye PKH67.29 PKH67+ cells were plated onto DAS104-4 cells and cultured for 3, 4, or 5 days. Cells were obtained, gated for live cells (PI−) and CD45 (hematopoietic cells), and PKH67-GL fluorescence intensity was analyzed by FACS. Fluorescence intensity of stem cells in the PKH67highregion was obtained by gating cells for high expression of PKH67 and analyzing the fluorescence intensity of these cells. Note that the fluorescence intensity of cells in the PKH67high region goes down with time, although at a slower rate than the bulk of cells.

Analysis of fluorescently labeled stem cells in vitro. linCD34+Sca-1+c-Kit+fetal liver stem cells were stained with the fluorescent dye PKH67.29 PKH67+ cells were plated onto DAS104-4 cells and cultured for 3, 4, or 5 days. Cells were obtained, gated for live cells (PI) and CD45 (hematopoietic cells), and PKH67-GL fluorescence intensity was analyzed by FACS. Fluorescence intensity of stem cells in the PKH67highregion was obtained by gating cells for high expression of PKH67 and analyzing the fluorescence intensity of these cells. Note that the fluorescence intensity of cells in the PKH67high region goes down with time, although at a slower rate than the bulk of cells.

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