CD164 is located on human chromosome 6q21. Somatic cell hybrids shown in Tables 3 and 4 were analyzed by PCR for the presence of the CD164 gene. (A) Ethidium bromide–stained gel of PCR products using the MGC24-Gp-F4/B4 primer pairs. This gel was Southern blotted and probed with CD164 cDNA probe C as indicated in (B). Partial metaphase spreads (C) showing localization of the BglII subclone of CD164 PAC1 to human chromosome band 6q21 (arrowed and shown on the idiogram).