Triple-color analysis of primitive markers on CD34⁺CD164(103B2/9E10 epitope)⁺ bone marrow cells. CD34⁺ bone marrow cells were labeled with 103B2/9E10 plus CD34-PerCP and with each of the antibody PE conjugates indicated before staining with FITC-F(ab)₂ goat anti-mouse IgG₃. Plots are two-color dot plot displays from a representative experiment of CD34 purified cells gated on forward and side scatter parameters (left top dot plot) and on PerCP-CD34⁺ fluorescence and side scatter (middle dot plot). The arbitrary gates indicated on the dot plots were determined with appropriate isotype-matched negative controls. Examples of the IgG₁/IgG₃ isotype negative controls (top right dot plot) and of CD164(103B2/9E10 epitope)/anti–IgG₃-FITC staining plus labeling with a nonbinding PE-conjugated anti–glycophorin A conjugate (bottom right dot plot) are indicated. Similar results were obtained for the IgG₁/IgG_2a isotype negative controls.