American Society of Hematology

Fig. 6.

$Fig. 6. PKC translocation in basophils stimulated with PMA and/or ionomycin. Basophils (purity: 82% ± 2%, n = 2 ) were incubated with or without PMA (1 ng/mL) and with or without ionomycin (0.1 μg/mL) for 15 minutes. Membrane and cytosol fractions were prepared, analyzed by Western blotting, and the bands quantified by digital imaging. Data are presented as mean ± range for two experiments. Histamine release for these samples is also plotted.$

PKC translocation in basophils stimulated with PMA and/or ionomycin. Basophils (purity: 82% ± 2%, n = 2 ) were incubated with or without PMA (1 ng/mL) and with or without ionomycin (0.1 μg/mL) for 15 minutes. Membrane and cytosol fractions were prepared, analyzed by Western blotting, and the bands quantified by digital imaging. Data are presented as mean ± range for two experiments. Histamine release for these samples is also plotted.

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