Fig. 2.
![Fig. 2. Translocation of PKC isozymes in basophils stimulated with PMA. Basophils (purity = 80% ± 3%, n = 4) were incubated with or without PMA (100 ng/mL) for 15 minutes. The membrane and cytosol fractions were subjected to Western blot analysis and the band intensities were quantified by digital imaging. The top panel shows a representative Western blots from one experiment and the lower panel shows the amount of each isozyme in the cytosol or membrane expressed as a percentage of either component divided by the sum of both components [eg, cytoscol/(cytosol + membrane)]. Data are presented as mean ± SEM for four experiments. * P < .05, ***P < .001 (paired t-test).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/4/10.1182_blood.v92.4.1206/5/blod41601002w.jpeg?Expires=1724236245&Signature=kT-UunPA3msGvONnEMM00cIIJ0s62SBx35RfaLnndRsZ4lkkbXw2JPHgQrTRU19UaFWFHjeGtsqI436Gvvucb8lPCAn6aw3tyNtOtRoWlW1HAQ0CaD~MWOf~AVf3uXqu6rrU8GtV1d3XnVs~nlTTJAbhzMo4cstG87hcT3ZkA5hYwBiX1bDIncf6p-H9YEH7ts7XTGUwE1sEF9DS3-6dqEKDckBROK~1f9NUQaa2M4mJtPxwkvDY-M4Lwzp84ZDTt4f7x8C~DkWwBIRSGiwmZVoaFw2Bc~owxckeEC7cHnyYBItR5CFpjqj9ZiH-5JmkBdPZZqpvty8kwbwR1N8-Tg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Translocation of PKC isozymes in basophils stimulated with PMA. Basophils (purity = 80% ± 3%, n = 4) were incubated with or without PMA (100 ng/mL) for 15 minutes. The membrane and cytosol fractions were subjected to Western blot analysis and the band intensities were quantified by digital imaging. The top panel shows a representative Western blots from one experiment and the lower panel shows the amount of each isozyme in the cytosol or membrane expressed as a percentage of either component divided by the sum of both components [eg, cytoscol/(cytosol + membrane)]. Data are presented as mean ± SEM for four experiments. * P < .05, ***P < .001 (paired t-test).
