Expression of PKC isozymes in basophils and contaminating cells. Basophils (purity: 98% to 99.4%) and contaminating cells (lymphocytes and monocytes) were lysed and subjected to Western blot analysis as described in Material and Methods. (MWM) Molecular weight marker (upper band: 97.4 kD, lower band: 68 kD). (R) Rat brain extract. (B) Human basophils. (C) Contaminating cells (lymphocytes and monocytes). The blots are representative of five experiments for PKC  and δ and, three experiments for the other isozymes. The band intensities were quantified by digital imaging. The data are expressed as the amount of each isozyme present in basophils expressed as a percent of the amount in contaminating cells (right panel). The data represent the mean ± SEM.