(A) Kinetics of appearance after TM injection of peritoneal cells and of adherent peritoneal cells that enter S phase in response to CSF-1. At various times after intraperitoneal injection of TM, peritoneal cell numbers were counted (□; see the Materials and Methods). Data are the mean values ± SEM and are pooled from 5 experiments, each with 8 mice. Also, at various times after intraperitoneal injection of TM, adherent cells were treated in vitro for 4 days with CSF-1 and [³H]-TdR (•; see the Materials and Methods). Autoradiography was used to measure the number of S-phase cells (see the Materials and Methods). Data are the mean values ± SEM from triplicate cultures and are from a representative experiment that was repeated a total of 3 times. (B) Kinetics of appearance of peritoneal M-CFCs. At various times after intraperitoneal injection of TM, M-CFCs were measured as agar colonies (1,000 cells plated; see the Materials and Methods). Data are plotted as the number of M-CFCs in the peritoneal cavity (▵) using the total cell numbers presented in (A) and as a percentage of the peritoneal cells (•; mean values ± SEM from triplicate cultures) and are from a representative experiment that was repeated a total of 3 times. When error bars are not presented, the errors are smaller than the size of the symbol.