Flow-assisted characterization of anti-TNF–treated GTS cultures on day 10 revealed the expansion of the CD14-dependent DC pathway. A representative experiment for each group is shown. For CD14 versus CD1a, N = 9, for all other combinations, N ≥ 3. The numbers in the upper right quadrants depict percent positive cells for the experiment shown, after subtracting negative controls. With the exception of the CD80 versus CD1a combination, all comparisons yielded statistically significant differences (P ≤ .03). The addition of nonimmune rabbit IgG to control GTS cultures did not alter the distribution of any of the markers.