Anti-TNF treatment on day 3 alters the growth pattern associated with DC development in GTS cultures. GTS and anti-TNF–treated GTS cultures were compared in parallel. Results represent the mean of multiple experiments. (A) Proliferation was measured by thymidine uptake, N = 3 to 8 for all days. (B) Cell content was assessed by hemacytometer-assisted cell counts, N = 7 to 16 for all days. The addition of nonimmune rabbit IgG to GTS cultures on day 3 did not alter the growth pattern.