Multiple forms of SHIP are present in the murine hematopoietic cell line, DA-ER. (A) A diagrammatic representation of SHIP and an indication of the regions used to generate anti–N-, anti–M-, and anti–C-antisera. (B) An anti-M immunoblot of anti-N (lane 1) and anti-M (lane 2) immunoprecipitates from IL-3–stimulated DA-ER cells. (C) The left panel is an anti-M immunoblot of anti-N or anti-C immunoprecipitates from IL-3–stimulated DA-ER cells and the right panel is a reprobing of the anti-N immunoprecipitated sample with anti-C. (D) An anti-N immunoblot of a total cell lysate from 1 × 10⁷ nucleated mouse bone marrow cells.