American Society of Hematology

Fig. 3.

$Fig. 3. GM-CSF and Epo enhance Bcl-3 nuclear translocation. Growth factor-induced TF-1 cells (1 × 107) were used for nuclear or cytoplasm separation. Twenty-five micrograms of nuclear or 20 μg of cytosolic protein extracts was loaded on each lane of a 12% polyacrylamide gel and subjected to Western blotting with anti–Bcl-3 antibody. As a control, 15 μg of nuclear or cytoplasmic extract was loaded on a 10% polyacrylamide gel to detect IκB. For GM-CSF–stimulated cells, 30 μg of nuclear or cytoplasmic extract was loaded on each lane of 12% gel and detection was with anti-E47 antibody as another control for quality of subcellular fractionation. Representative results are shown from 3 independent experiments.$

GM-CSF and Epo enhance Bcl-3 nuclear translocation. Growth factor-induced TF-1 cells (1 × 10⁷) were used for nuclear or cytoplasm separation. Twenty-five micrograms of nuclear or 20 μg of cytosolic protein extracts was loaded on each lane of a 12% polyacrylamide gel and subjected to Western blotting with anti–Bcl-3 antibody. As a control, 15 μg of nuclear or cytoplasmic extract was loaded on a 10% polyacrylamide gel to detect IκB. For GM-CSF–stimulated cells, 30 μg of nuclear or cytoplasmic extract was loaded on each lane of 12% gel and detection was with anti-E47 antibody as another control for quality of subcellular fractionation. Representative results are shown from 3 independent experiments.

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