Fig. 10.
CD23 ligation reduces the percentage of annexin V-labeled B-CLL cells. Freshly collected leukemic cells from two patients were incubated for 48 hours in the presence of medium alone (upper histograms) or containing anti-CD23 MoAb (MoAb 135, 10 μg/mL) (lower histograms), then labeled with FITC-annexin V. FACS analysis was performed as previously on the global cell population, using an FITC-labeled goat anti-rabbit Ig F(ab′)2 (black line) as a control of nonspecific labeling for FITC-Annexin V (gray line).
