Suppression of TNF-induced nuclear localization of Rel proteins in RCC patients. T cells from a normal donor and an RCC patient (VA) were stimulated with 10 ng/mL of TNF for the times indicated. The nuclear and cytoplasmic extracts were then obtained and subjected to immunoblotting using antibodies to c-Rel, RelA, NFκB1 (p50), and IκB. Representative data are presented and similar results were obtained in 2 additional experiments.