Northern blot analysis of the expression of ILs in MS-10 cells or their receptors in HB-1 cells by the coculture of MS-10 and HB-1 cells. (A) Result of hybridization with the³²P-labeled probes for IL-1α and IL-11. A total of 15 μg of total RNA of each sample was loaded in each lane. Each lane represents HB-1 cells separated from MS-10 cells for 5 days (lane 3); HB-1 cells cocultured with MS-10 cells (lane 4); HB-1 cells separated from MS-10 cells for 5 days then reseeded on MS-10 cells again and harvested 5 days later (lane 5); or cultured in the presence of 10 U/mL IL-1α and harvested 5 days later ( lane 6 ); MS-10 cells cocultured with HB-1 cells (lane 7); and MS-10 cells alone (lane 8). LPS-stimulated MS-5 cells were used as positive control (lane 2). MS-5 cells were used as negative control (lane 1). The level of β-actin is shown as control. (B) Result of hybridization with the³²P-labeled probes for IL-1RI and IL-11R. A total of 15 μg of total RNA from each sample was loaded in each lane. Each lane represents HB-1 cells separated from MS-10 cells for 5 days (lane 3); HB-1 cells cocultured with MS-10 cells (lane 4); HB-1 cells separated from MS-10 cells for 5 days then reseeded on MS-10 cells again and harvested 5 days later (lane 5); cultured in the presence of 10 U/mL IL-1α and harvested 5 days later (lane 6); MS-10 cells cocultured with HB-1 cells (lane 7); MS-10 cells alone (lane 8). C3H/HeN spleen cells and NIH3T3 cells were used as control. The level of β-actin is shown as control.